Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel

Neuron. 1991 Sep;7(3):471-83. doi: 10.1016/0896-6273(91)90299-f.


A cDNA clone encoding a K+ channel polypeptide with 72% amino acid sequence identity to Drosophila Shal was isolated from rat hippocampus. Functional expression of the cDNA in Xenopus oocytes generated 4-amino-pyridine-sensitive K+ channels displaying rapid inactivation kinetics. The fastest component of inactivation was slowed by the deletion of 3 basic residues in the amino-terminal region. Northern blots revealed that the mRNA encoding this K+ channel polypeptide was expressed at a similar level in the brain and in the heart. In situ hybridization revealed that the mRNA encoding this K+ channel appeared concentrated in the hippocampus, dentate gyrus, and habenular nucleus in the brain. Thus, this K+ channel polypeptide is likely to form some of the A-type K+ channels expressed in the mammalian nervous system and heart.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Gene Expression
  • Heart / physiology
  • Hippocampus / physiology*
  • Ion Channel Gating
  • Membrane Potentials
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • Potassium Channels / genetics*
  • Potassium Channels / physiology
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment


  • Oligodeoxyribonucleotides
  • Potassium Channels
  • RNA, Messenger

Associated data

  • GENBANK/M67516
  • GENBANK/S61932
  • GENBANK/S61935
  • GENBANK/S64320
  • GENBANK/S70258
  • GENBANK/S70261
  • GENBANK/S70262
  • GENBANK/X57453
  • GENBANK/X57454
  • GENBANK/X57455