Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies

Nat Methods. 2008 Jun;5(6):531-3. doi: 10.1038/nmeth.1203. Epub 2008 Apr 13.


A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings. Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the absence of anesthetics.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Axons / pathology*
  • Axotomy / methods*
  • Behavior, Animal
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / metabolism
  • Equipment Design
  • Microfluidic Analytical Techniques
  • Microfluidics
  • Models, Biological
  • Nanotechnology / methods*
  • Nerve Regeneration*
  • Time Factors


  • Caenorhabditis elegans Proteins