Effects of fluorides on Candida albicans

Oral Dis. 2008 May;14(4):296-301. doi: 10.1111/j.1601-0825.2007.01385.x.


Aims: To assess whether a short exposure of Candida albicans to commonly used fluorides would affect growth, cell surface hydrophobicity, and adherence to buccal epithelial cells.

Methods: Candida albicans ATCC 90028 and 11 clinical isolates were used. Minimal inhibitory concentrations (MICs) of sodium fluoride (NaF) and of an amine fluoride / stannous fluoride combination (AmF / SnF2) were determined. Yeasts were exposed to MICs of tested agents for 1 h. Subsequently, their growth was recorded spectrophotometrically. Their cell surface hydrophobicity was assessed with n-hexadecane. Adherence to buccal epithelial cells was determined microscopically. Phosphate buffered saline (PBS) and chlorhexidine digluconate (CHX) served as controls. All results were analyzed by one-way ANOVA.

Results: MICs of AmF / SnF(2) and CHX varied between 1 and 4 microg ml(-1), whereas those of NaF were 15 000 microg ml(-1). Statistically significant growth inhibition was detected after AmF / SnF(2) (OD(24 h) +/- SD 0.457 +/- 0.059) and CHX (0.175 +/- 0.065) in comparison with PBS (0.925 +/- 0.087) and NaF (0.813 +/- 0.081). All strains demonstrated uniform behavior. Only minor changes in cell surface hydrophobicity and adherence to buccal epithelial cells (BEC) were detected.

Conclusion: Growth inhibition of AmF / SnF(2) was comparable with that of CHX whereas NaF had a weaker effect. Exposure to the fluorides did not seem to alter the cell surface hydrophobicity nor adherence to BEC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amines / pharmacology*
  • Analysis of Variance
  • Antifungal Agents / pharmacology*
  • Candida albicans / drug effects*
  • Cell Adhesion / drug effects
  • Cells, Cultured
  • Cheek
  • Chlorhexidine / pharmacology
  • Drug Combinations
  • Epithelial Cells
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Microbial Sensitivity Tests
  • Mouth Mucosa / cytology
  • Sodium Fluoride / pharmacology*
  • Tin Fluorides / pharmacology*


  • Amines
  • Antifungal Agents
  • Drug Combinations
  • Tin Fluorides
  • amine fluoride gel
  • Sodium Fluoride
  • Chlorhexidine