Rapid isolation of host-independent Bdellovibrio bacteriovorus

J Microbiol Methods. 2008 Jun;73(3):279-81. doi: 10.1016/j.mimet.2008.02.023. Epub 2008 Mar 7.

Abstract

A new method of isolating host-independent Bdellovibrio bacteriovorus has been developed. Filtered suspensions of host-dependent cells are dropped in small volumes onto 0.2 microm membranes laid on rich media agar. Significant growth is observed within 1-2 days; these cells were confirmed to be B. bacteriovorus using microscopic observations and PCR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriological Techniques / methods*
  • Bdellovibrio / cytology
  • Bdellovibrio / genetics
  • Bdellovibrio / growth & development*
  • Bdellovibrio / isolation & purification*
  • DNA, Bacterial / genetics
  • Microscopy
  • Polymerase Chain Reaction

Substances

  • DNA, Bacterial