Rat DNA sequence associated with a complex form of DNA polymerase alpha in nonregenerating liver interacts with a ubiquitous transcription/replication factor Oct-1

Biomed Sci. 1991;2(6):595-600.


A ubiquitous mammalian transcription factor, Oct-1 (also known as OTF-1, NF-A1, OBP100, or NFIII), stimulates the initiation of replication of adenovirus DNA, and may also be involved in the activation of some chromosomal replication origins. If this is true, binding sites for Oct-1 should be present within regions responsible for the initiation of DNA replication. In this study such a binding site has been identified within a 340bp fragment that was originally isolated from a minor fraction of DNA associated with a complexed form of DNA polymerase alpha from nonregenerating rat liver, and which shows autonomous replication sequence activity in a transient transfection assay. Northern blot analysis was used to show that Oct-1 mRNA is induced in regenerating rat liver 6-14 h after hepatectomy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • DNA / metabolism*
  • DNA Polymerase II / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Deoxyribonuclease I / metabolism
  • Hepatectomy
  • Host Cell Factor C1
  • Liver / enzymology
  • Liver / metabolism*
  • Liver Regeneration
  • Molecular Sequence Data
  • Octamer Transcription Factor-1
  • Rats
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • Host Cell Factor C1
  • Octamer Transcription Factor-1
  • Pou2f1 protein, rat
  • Transcription Factors
  • DNA
  • DNA Polymerase II
  • Deoxyribonuclease I