A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains

J Microbiol Methods. 2008 Jun;73(3):273-5. doi: 10.1016/j.mimet.2008.02.022. Epub 2008 Mar 6.


Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxI-family promoters independently from AHLs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl-Butyrolactones / metabolism
  • Artificial Gene Fusion
  • Biosensing Techniques / methods*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Enterobacter aerogenes / physiology*
  • Molecular Sequence Data
  • Plasmids
  • Quorum Sensing*
  • Sequence Analysis, DNA
  • beta-Galactosidase / biosynthesis
  • beta-Galactosidase / genetics


  • Acyl-Butyrolactones
  • DNA, Bacterial
  • beta-Galactosidase

Associated data

  • GENBANK/AM933750
  • GENBANK/AM933751