Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities

Nucleic Acids Symp Ser. 1991;(25):79-80.

Abstract

Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Cattle
  • DNA Polymerase III / antagonists & inhibitors*
  • Dideoxynucleotides
  • HIV / drug effects
  • Male
  • Thymine Nucleotides / pharmacology*
  • Zidovudine / analogs & derivatives*
  • Zidovudine / pharmacology

Substances

  • Antiviral Agents
  • Dideoxynucleotides
  • Thymine Nucleotides
  • Zidovudine
  • zidovudine triphosphate
  • DNA Polymerase III
  • 2',3'-dideoxythymidine triphosphate