Meiosis I is established through division-specific translational control of a cyclin

Cell. 2008 Apr 18;133(2):280-91. doi: 10.1016/j.cell.2008.02.032.

Abstract

In budding yeast, key meiotic events such as DNA replication, recombination, and the meiotic divisions are controlled by Clb cyclin-dependent kinases (Clb-CDKs). Using a novel synchronization procedure, we have characterized the activity of these Clb-CDKs and observed a surprising diversity in their regulation during the meiotic divisions. Clb1-CDK activity is restricted to meiosis I, and Clb3-CDK activity to meiosis II, through 5'UTR-mediated translational control of its transcript. The analysis of cells inappropriately producing Clb3-CDKs during meiosis I furthermore defines Clb3 as an inhibitor of the meiosis I chromosome segregation program. Our results demonstrate an essential role for Clb-CDK regulation in establishing the meiotic chromosome segregation pattern.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / metabolism
  • Chromatids / metabolism
  • Cyclin B
  • Cyclins / metabolism*
  • Meiosis*
  • Protein Biosynthesis*
  • Protein Kinases / metabolism
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • 5' Untranslated Regions
  • CLB1 protein, S cerevisiae
  • CLB3 protein, S cerevisiae
  • Cyclin B
  • Cyclins
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases