Imaging of reactive oxygen species and nitric oxide in vivo in plant tissues

Methods Enzymol. 2008;440:397-409. doi: 10.1016/S0076-6879(07)00825-7.


During the last decades there has been a growing interest in the study of reactive oxygen species (ROS) and nitric oxide (NO) production in plant tissues and their role in signaling and cellular response to biotic and abiotic stress conditions. Despite growing molecular data on this subject, less attention has been paid to the topological distribution of ROS and NO production in plant tissues. Knowledge of the contribution of different cells to the accumulation of ROS and NO is important to get deeper insights into the cellular response of plants to adverse conditions. This chapter focuses on the imaging of ROS and NO accumulation in vivo in plant tissues by confocal laser microscopy using specific fluorescent probes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Fluorescent Dyes / metabolism
  • Microscopy / methods*
  • Microscopy, Confocal / methods
  • Nitric Oxide / metabolism*
  • Plants / metabolism*
  • Reactive Oxygen Species / metabolism*


  • Fluorescent Dyes
  • Reactive Oxygen Species
  • Nitric Oxide