Histomorphometric evaluation of the effects of zoledronic acid on mandibular distraction osteogenesis in rabbits

J Oral Maxillofac Surg. 2008 May;66(5):905-10. doi: 10.1016/j.joms.2007.12.004.


Purpose: To assess the effect of systemic administration of zoledronic acid (ZA) on mineralization of newly formed bone and to determine strain-related osteoporosis on surrounding bone during lengthening of immature rabbit mandible.

Materials and methods: Eighteen New Zealand white rabbits were divided randomly into 2 groups, and bone lengthening was carried out in the left portion of the mandible through distraction osteogenesis with a rate of 0.5 mm every 12 hours for 5 days. The experimental group was administered 0.1 mg/kg ZA intravenously. The control group was given saline infusion only during operation. All animals were sacrificed at the end of the 28-day consolidation period. The mandibles of all animals were removed and regenerate was evaluated. Osteoblasts, osteoclasts, collagen fibers, and fibroblasts were marked within 0.1-mm(2) area and newly formed bone area was measured within 0.5-mm(2) area. All data were analyzed using Mann-Whitney U test.

Results: Although irregular bone destruction spots were seen in the control group, the experimental group showed regular ossification areas and significant difference between osteoblast and osteoclast numbers (P < .05). In the regenerate zone, there was considerable difference between the 2 groups in terms of osteoblast, osteoclast, and collagen amounts (P < .05). Additionally, newly formed bone areas and fibroblast count were higher in experimental group.

Conclusions: The results of this study showed that ZA had positive effects on the new bone formation, which may potentially shorten the consolidation period.

MeSH terms

  • Animals
  • Bone Density Conservation Agents / pharmacology*
  • Bone Regeneration / drug effects*
  • Calcification, Physiologic / drug effects
  • Cell Proliferation / drug effects
  • Collagen / biosynthesis
  • Dental Stress Analysis
  • Diphosphonates / pharmacology*
  • Fibroblasts / drug effects
  • Imidazoles / pharmacology*
  • Male
  • Mandible / surgery
  • Mandibular Advancement / adverse effects
  • Mandibular Advancement / methods*
  • Osteoblasts / drug effects
  • Osteoclasts / drug effects
  • Osteogenesis, Distraction* / adverse effects
  • Osteoporosis / etiology
  • Osteoporosis / prevention & control
  • Rabbits
  • Random Allocation
  • Zoledronic Acid


  • Bone Density Conservation Agents
  • Diphosphonates
  • Imidazoles
  • Zoledronic Acid
  • Collagen