Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207. Epub 2008 Apr 20.


Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET-based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Caspase 3 / metabolism
  • Fluorescence Resonance Energy Transfer / methods*
  • Green Fluorescent Proteins
  • HeLa Cells
  • Humans
  • Image Processing, Computer-Assisted
  • Luminescent Proteins*
  • Plant Proteins / chemistry


  • Luminescent Proteins
  • Plant Proteins
  • Green Fluorescent Proteins
  • Caspase 3