Insulin-like growth factor I (IGF-I) from cow colostrum: purification and characterization

Growth Regul. 1991 Jun;1(2):56-61.


Insulin-like growth factor I (IGF-I) has been purified from defatted cow colostrum. The purification procedure involved cation exchange chromatography on CM Affigel blue, acid gel-filtration and two HPLCs. This purification process constitutes a significant improvement, in terms of yield and rapidity, over the previously reported procedures. Purified IGF-I was found to be 95% pure by N-terminal amino acid sequence analysis. After isolation of peptides from a digest of IGF-I obtained using staphylococcus aureus protease, the complete sequence was established and found to be identical to that of bovine or human plasma IGF-I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Colostrum / chemistry*
  • Female
  • Insulin-Like Growth Factor I / chemistry
  • Insulin-Like Growth Factor I / isolation & purification*


  • Insulin-Like Growth Factor I