Using BiblioSpec for creating and searching tandem MS peptide libraries

Curr Protoc Bioinformatics. 2007 Dec:Chapter 13:13.7.1-13.7.12. doi: 10.1002/0471250953.bi1307s20.


BiblioSpec is a software package for creating and searching libraries of tandem MS peptide spectra. Library searching provides a quick method for making peptide-spectrum matches by comparing a query spectrum to a collection of reference spectra of known peptide sequence. Pre-assembled libraries for several model organisms can be used as the basis of a search or custom libraries can easily be assembled. The protocols in this unit describe installing BiblioSpec, searching libraries, and creating custom libraries.

MeSH terms

  • Database Management Systems
  • Databases, Protein*
  • Information Storage and Retrieval / methods
  • Mass Spectrometry / methods*
  • Peptide Library*
  • Peptide Mapping / methods*
  • Proteins / chemistry*
  • Proteins / ultrastructure
  • Sequence Analysis, Protein / methods*
  • Software*
  • User-Computer Interface


  • Peptide Library
  • Proteins