Validation of tandem mass spectrometry database search results using DTASelect

Curr Protoc Bioinformatics. 2007 Jan;Chapter 13:Unit 13.4. doi: 10.1002/0471250953.bi1304s16.

Abstract

DTASelect provides a means by which complex SEQUEST results can be filtered, organized, and viewed. A single sample may produce tens of thousands of tandem mass spectra. Manually perusing and selecting SEQUEST matches among such a mass of data carries a risk of inconsistency. DTASelect allows the user to set complex criteria for acceptance or rejection of individual spectrum results. It also features rules for dealing with multiple, identical peptide matches and for removing proteins that are insufficiently evidenced. It provides its sorted and filtered summary as HTML and text documents for easy review and also offers several auxiliary reports. DTASelect is a powerful tool for automatic analysis of complex mixture tandem mass spectrometry.

Publication types

  • Evaluation Study
  • Validation Study

MeSH terms

  • Amino Acid Sequence
  • Database Management Systems*
  • Databases, Protein*
  • Information Storage and Retrieval / methods*
  • Mass Spectrometry / methods*
  • Molecular Sequence Data
  • Peptide Mapping / methods*
  • Proteins / chemistry*
  • Sequence Analysis, Protein / methods*

Substances

  • Proteins