Abstract
This unit describes a protocol to obtain milligram amounts of enzymatically active pure recombinant caspases. Specific details for the expression, purification of caspase-3, -6, -7, -8, -9 and -10 are discussed along with strategies to obtain particular forms (e.g., the zymogen) of some of them.
MeSH terms
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Amino Acid Chloromethyl Ketones / metabolism
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Animals
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Biological Assay / methods*
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Caspases* / genetics
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Caspases* / isolation & purification
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Caspases* / metabolism
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Cysteine Proteinase Inhibitors / metabolism
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Enzyme Activation
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Humans
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Isoenzymes* / genetics
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Isoenzymes* / isolation & purification
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Isoenzymes* / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Substrate Specificity
Substances
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Amino Acid Chloromethyl Ketones
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Cysteine Proteinase Inhibitors
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Isoenzymes
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Recombinant Proteins
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benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
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Caspases