Overview of affinity tags for protein purification

Curr Protoc Protein Sci. 2004 Sep;Chapter 9:9.9.1-9.9.19. doi: 10.1002/0471140864.ps0909s36.

Abstract

Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, and for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.

Publication types

  • Review

MeSH terms

  • Affinity Labels*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification*

Substances

  • Affinity Labels
  • Recombinant Proteins