Stereomicroscopic fluorescence imaging of head and neck cancer xenografts targeting CD147

Cancer Biol Ther. 2008 Jul;7(7):1063-70. doi: 10.4161/cbt.7.7.6109. Epub 2008 Apr 14.


Purpose: To demonstrate that systemically administered fluorescently labeled anti-CD147 antibody can detect head and neck squamous cell carcinoma xenografts in vivo.

Experimental design: In vivo immunodeficient murine model.

Results: Peak tumor fluorescence was visualized by near infrared stereomicroscopy in SCC-1 tumors at 24 hours after systemic injection of anti-CD147:Cy5.5 bioconjugate. SCC-1 xenografts demonstrated significantly higher fluorescent intensity after administration of CD147:Cy5.5 (48 au, p < 0.0001) compared to IgG1k:Cy5.5 isotype control antibody (9 au). FaDu tumors overexpressing CD147 (FaDu/E) demonstrated higher fluorescence (53 au) compared to control vector transfected cells (FaDu, 33 au, p < 0.0001) which was higher than CD147 knockdown cells (FaDu/siE, 5 au, p < 0.0001).

Methods: To determine if fluorescently labeled anti-CD147 antibody was specific for tumors in vivo, anti-CD147 and non-specific IgG1k antibody were labeled with a near infrared fluorophore (Cy5.5) and administered systemically to immunodeficient mice bearing SCC-1 xenografts. Imaging was performed over a 72 hour period using brightfield and fluorescent (685-735 nm) stereomicroscopy. To determine if fluorescence varied with receptor expression, SCID mice were xenografted with cell lines expressing variable amounts of CD147: FaDu (control vector transfected), FaDu/siE (siRNA CD147 knockdown) or FaDu/E (CD147 overexpressing) cells.

Conclusions: This data suggests fluorescently labeled anti-CD147 may have clinical utility in detection of HNSCC.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basigin / biosynthesis*
  • Cell Line, Tumor
  • Contrast Media / pharmacology
  • Equipment Design
  • Head and Neck Neoplasms / metabolism*
  • Humans
  • Immunoglobulin G / chemistry
  • Mice
  • Mice, SCID
  • Microscopy / methods
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Neoplasm Transplantation
  • Skin / metabolism


  • Contrast Media
  • Immunoglobulin G
  • Basigin