Characterization of mouse hematopoietic stem and progenitor cells

doi:10.1002/0471142735.im22b02s80

Comparative Study

. 2008 Feb:Chapter 22:22B.2.1-22B.2.31.

doi: 10.1002/0471142735.im22b02s80.

Characterization of mouse hematopoietic stem and progenitor cells

Cindy L Miller¹, Brad Dykstra², Connie J Eaves³

Affiliations

¹ StemCell Technologies, Vancouver, British, Columbia.
² University Medical Centre Groningen, Groningen, The Netherlands.
³ Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia.

PMID: 18432636
DOI: 10.1002/0471142735.im22b02s80

Comparative Study

Characterization of mouse hematopoietic stem and progenitor cells

Cindy L Miller et al. Curr Protoc Immunol. 2008 Feb.

. 2008 Feb:Chapter 22:22B.2.1-22B.2.31.

doi: 10.1002/0471142735.im22b02s80.

Authors

Cindy L Miller¹, Brad Dykstra², Connie J Eaves³

Affiliations

¹ StemCell Technologies, Vancouver, British, Columbia.
² University Medical Centre Groningen, Groningen, The Netherlands.
³ Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia.

PMID: 18432636
DOI: 10.1002/0471142735.im22b02s80

Abstract

The unit describes functional assays for the quantification of mouse hematopoietic stem cells and progenitor cells. The competitive repopulating unit (CRU) assay detects transplantable mouse hematopoietic stem cells with the capacity to regenerate all of the blood cell lineages for extended time periods in vivo. The long-term culture-initiating cell (LTC-IC) assay, founded on the bone marrow long-term culture system, measures primitive hematopoietic progenitors based on their capacity to produce myeloid progeny for at least four weeks. Colony-forming cell (CFC) assays, performed in semisolid medium cultures to assess mouse pre-B, megakaryocyte, erythroid, granulocyte-monocyte, and multipotential hematopoietic progenitors are also described. These assays are powerful tools for evaluating human stem cell (HSC) and progenitor content in various hematopoietic tissues, during development as well as in the adult animal, and in cell populations manipulated ex vivo.

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References

Literature Cited

1. Audet, J., Miller, C.L., Rose-John, S., Piret, J.M., and Eaves, C.J. 2001. Distinct role of gp130 activation in promoting self-renewal divisions by mitogenically stimulated murine hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 98:1757-1762.
1. Benveniste, P., Cantin, C., Hyam, D., and Iscove, N.N. 2003. Hematopoietic stem cells engraft in mice with absolute efficiency. Nat. Immunol. 4:708-713.
1. Bowie, M.B., McKnight, K.D., Kent, D.G., McCaffrey, L., Hoodless, P.A., and Eaves, C.J. 2006. Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J. Clin. Invest. 116:2808-2816.
1. Bryder, D., Sasaki, Y., Johan Borge, O., and Jacobsen, S-E.W. 2004. Deceptive multilineage reconstitution analysis of mice transplanted with hematopoietic stem cells and implications for assessment of stem cell numbers and lineage potentials. J. Immunol. 172:1548-1552.
1. Bryder, D., Rossi, D.J., and Weissman, I.L. 2006. Hematopoietic stem cells: The paradigmatic tissue-specific stem cell. Am. J. Pathol. 169:338-346.

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[1] Audet, J., Miller, C.L., Rose-John, S., Piret, J.M., and Eaves, C.J. 2001. Distinct role of gp130 activation in promoting self-renewal divisions by mitogenically stimulated murine hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 98:1757-1762.

[2] Audet, J., Miller, C.L., Rose-John, S., Piret, J.M., and Eaves, C.J. 2001. Distinct role of gp130 activation in promoting self-renewal divisions by mitogenically stimulated murine hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 98:1757-1762.

[3] Benveniste, P., Cantin, C., Hyam, D., and Iscove, N.N. 2003. Hematopoietic stem cells engraft in mice with absolute efficiency. Nat. Immunol. 4:708-713.

[4] Benveniste, P., Cantin, C., Hyam, D., and Iscove, N.N. 2003. Hematopoietic stem cells engraft in mice with absolute efficiency. Nat. Immunol. 4:708-713.

[5] Bowie, M.B., McKnight, K.D., Kent, D.G., McCaffrey, L., Hoodless, P.A., and Eaves, C.J. 2006. Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J. Clin. Invest. 116:2808-2816.

[6] Bowie, M.B., McKnight, K.D., Kent, D.G., McCaffrey, L., Hoodless, P.A., and Eaves, C.J. 2006. Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J. Clin. Invest. 116:2808-2816.

[7] Bryder, D., Sasaki, Y., Johan Borge, O., and Jacobsen, S-E.W. 2004. Deceptive multilineage reconstitution analysis of mice transplanted with hematopoietic stem cells and implications for assessment of stem cell numbers and lineage potentials. J. Immunol. 172:1548-1552.

[8] Bryder, D., Sasaki, Y., Johan Borge, O., and Jacobsen, S-E.W. 2004. Deceptive multilineage reconstitution analysis of mice transplanted with hematopoietic stem cells and implications for assessment of stem cell numbers and lineage potentials. J. Immunol. 172:1548-1552.

[9] Bryder, D., Rossi, D.J., and Weissman, I.L. 2006. Hematopoietic stem cells: The paradigmatic tissue-specific stem cell. Am. J. Pathol. 169:338-346.

[10] Bryder, D., Rossi, D.J., and Weissman, I.L. 2006. Hematopoietic stem cells: The paradigmatic tissue-specific stem cell. Am. J. Pathol. 169:338-346.

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Characterization of mouse hematopoietic stem and progenitor cells

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Characterization of mouse hematopoietic stem and progenitor cells

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