T cell depletion by cytotoxic elimination

Curr Protoc Immunol. 2001 May:Chapter 3:Unit 3.4. doi: 10.1002/0471142735.im0304s00.

Abstract

This unit describes the complete removal of T cells from lymphocyte preparations based on the presence of the glycoprotein Thy-1 on the cell surface of T lymphocytes. As in UNIT 3.3, cytotoxic elimination is employed; however, Thy-1-specific antibodies are used rather than MHC class II-specific antibodies so that T cells are eliminated rather than B cells and accessory cells. Murine T cells can be further fractionated into subpopulations based on their expression of cell differentiation markers CD4 (Leu3, L3T4, T4) and CD8 (Leu2, Lyt2,3). A protocol in this unit describes removal of T cell subsets by cytotoxic elimination using CD-specific antibodies. An alternate but technically more demanding method of enriching T cell subsets is the panning procedure described in UNIT 3.5.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • CD4 Antigens / immunology
  • CD8 Antigens / immunology
  • Cell Separation / methods*
  • Cytotoxicity, Immunologic
  • Mice
  • T-Lymphocytes* / classification
  • T-Lymphocytes* / immunology
  • Thy-1 Antigens / immunology

Substances

  • Antibodies, Monoclonal
  • CD4 Antigens
  • CD8 Antigens
  • Thy-1 Antigens