An Arabidopsis thaliana G-box-binding protein similar to the wheat leucine zipper protein identified as HBP-1

Symp Soc Exp Biol. 1991;45:211-8.

Abstract

G-box (CCACGTGG) like sequences are present in a variety of plant promoters and in many cases they have been demonstrated to be required for maximal expression of the corresponding gene. A nuclear protein, GBF, interacts specifically with the G-box motif of several RBCS and CAB promoters. Here we describe the isolation of a cDNA from Arabidopsis thaliana that encodes a protein, designated GBF-1, with DNA binding properties similar to GBF. GBF-1 is characterized by a basic/leucine zipper motif which is strikingly similar to the wheat protein identified as HBP-1. GBF-1 also interacts with an oligonucleotide derived from the wheat histone 3 promoter containing the binding site (hexamer, TGACGT) for HBP-1. This DNA element also contains a G-box-like motif, modification of which results in loss in binding of GBF-1.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Basic-Leucine Zipper Transcription Factors
  • Binding Sites
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • G-Box Binding Factors
  • Leucine Zippers / genetics*
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Promoter Regions, Genetic
  • Sequence Homology, Amino Acid
  • Transcription Factors*

Substances

  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • G-Box Binding Factors
  • Nuclear Proteins
  • Plant Proteins
  • Transcription Factors
  • histone DNA binding protein-1
  • DNA