Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Mar;4(3):205-12.
doi: 10.1039/b715110c. Epub 2008 Jan 8.

Normalization of Full-Length Enriched cDNA

Affiliations

Normalization of Full-Length Enriched cDNA

Ekaterina A Bogdanova et al. Mol Biosyst. .

Abstract

Analysis of rare messages in cDNA libraries is extremely difficult due to the substantial variations in the abundance of different transcripts in cells and tissues. Therefore, for rare transcript searches and analyses, the generation of equalized (normalized) cDNA is essential. Several cDNA normalization methods have been developed since 1990. A number of these methods have been optimized for the normalization of full-length enriched cDNA, and used in various applications, including transcriptome analysis and functional screening of cDNA libraries. One such procedure (named DSN-normalization) is based on the unique properties of duplex-specific nuclease (DSN) from kamchatka crab and allows the generation of normalized cDNA libraries with a high gene discovery rate.

Similar articles

See all similar articles

Cited by 21 articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback