Flow cytometry of bacterial membrane potential and permeability

Methods Mol Med. 2008;142:175-86. doi: 10.1007/978-1-59745-246-5_14.

Abstract

This chapter describes reliable flow cytometric methods for assessment of two important physiologic characteristics of bacteria, membrane potential and membrane permeability, which can provide indications of the effects of antimicrobial agents on microorganisms.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Physiological Phenomena / drug effects
  • Carbocyanines / metabolism
  • Cell Membrane Permeability / drug effects*
  • Flow Cytometry / methods*
  • Fluorescent Dyes / metabolism
  • Membrane Potentials / drug effects*
  • Mycobacterium smegmatis / drug effects
  • Mycobacterium smegmatis / physiology
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / physiology

Substances

  • Anti-Bacterial Agents
  • Carbocyanines
  • Fluorescent Dyes
  • TO-PRO-3
  • 3,3'-diethyloxacarbocyanine