Up-regulated HIF-1alpha is involved in the hypoxic tolerance induced by hyperbaric oxygen preconditioning

Brain Res. 2008 May 30;1212:71-8. doi: 10.1016/j.brainres.2008.03.027. Epub 2008 Mar 27.


Hyperbaric oxygen preconditioning (HBO-PC) has been shown to be effective in preventing hypoxic injuries in many animal models. The aim of the present study was to examine the hypoxic tolerance induced by HBO-PC and to explore the role of hypoxia-inducible factor-1alpha (HIF-1alpha) in a global hypoxia model. Male mice received HBO-PC before hypoxia exposure and swimming. HBO-PC significantly prolonged the survival time and the tolerance time of swimming under normobaric hypoxia. HBO-PC increased the protein content of HIF-1alpha and erythropoietin (EPO) in the cerebral cortex and hippocampus and prevented the changes of blood brain barrier (BBB) permeability and brain edema caused by hypoxia exposure. The results suggested that HBO-PC induced hypoxic tolerance in mice via up-regulation of HIF-1alpha and its downstream genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Behavior, Animal
  • Brain Edema / etiology
  • Cerebral Cortex / metabolism
  • Disease Models, Animal
  • Erythropoietin / metabolism
  • Hippocampus / metabolism
  • Hyperbaric Oxygenation*
  • Hypoxia / complications
  • Hypoxia / metabolism*
  • Hypoxia / mortality
  • Hypoxia / pathology
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
  • Ischemic Preconditioning*
  • Male
  • Mice
  • Mice, Inbred Strains
  • Survival Analysis
  • Time Factors
  • Up-Regulation / physiology*


  • Hif1a protein, mouse
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Erythropoietin