Effects of antioxidants on calcium influx through TRPM2 channels in transfected cells activated by hydrogen peroxide

J Neurol Sci. 2008 Jul 15;270(1-2):152-8. doi: 10.1016/j.jns.2008.03.003. Epub 2008 Apr 28.

Abstract

Melastatin-like transient receptor potential 2 (TRPM2) channel is a redox sensitive Ca(2+)-permeable cation channel that can be gated by H(2)O(2) binding to the channel's enzymatic Nudix domain. Since the mechanisms that lead to TRPM2 action in response to H(2)O(2) are not understood, we examined the effects of various antioxidants on H(2)O(2)-induced TRPM2 cation channel currents in transfected Chinese hamster ovary (CHO) cells. The CHO cells were transfected with cDNA coding for TRPM2. Membrane currents were measured with the conventional whole cell patch-clamp technique. The intracellular solution contained ethylenediamine tetraacetic acid (EDTA) as a chelator for Ca(2+) and heavy metal ions instead of ethylene glycol tetraacetic acid (EGTA). Moreover, we chose an intracellular Ca(2+) concentration calculated to be in the range of 1 microM. H(2)O(2) (10 mM) was added extracellularly to the bath chamber. With these conditions, we were able to evoke TRPM2 currents consistently with H(2)O(2). We next tested whether vitamins C and E or glutathione (GSH) would prevent or attenuate the induction of TRPM2 currents by H(2)O(2) when applied extracellularly or intracellularly. Unexpectedly, administration of these antioxidants did not inhibit activation of TRPM2 by H(2)O(2). In conclusion, TRPM2 channels were constitutively activated by H(2)O(2) although we could not detect any inhibitory effect of the antioxidants on H(2)O(2)-induced TRPM2 cation channel currents in CHO cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology
  • CHO Cells / drug effects
  • CHO Cells / metabolism
  • Calcium / metabolism*
  • Chelating Agents / pharmacology
  • Clusterin / genetics
  • Clusterin / physiology*
  • Cricetinae
  • Cricetulus
  • Drug Interactions
  • Edetic Acid / pharmacology
  • Electric Stimulation / methods
  • Glutathione / pharmacology
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Hydrogen Peroxide / pharmacology*
  • Membrane Potentials / drug effects*
  • Membrane Potentials / physiology
  • Mutation
  • Patch-Clamp Techniques / methods
  • Transfection / methods
  • Vitamin E / pharmacology

Substances

  • Antioxidants
  • Chelating Agents
  • Clusterin
  • Vitamin E
  • Green Fluorescent Proteins
  • Edetic Acid
  • Hydrogen Peroxide
  • Glutathione
  • Ascorbic Acid
  • Calcium