Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 1 (1), 31-7

Coinjection Strategy for Visual Identification of Transgenic Mice

Affiliations

Coinjection Strategy for Visual Identification of Transgenic Mice

P A Overbeek et al. Transgenic Res.

Abstract

Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a gamma-glutamyl transferase (gamma GT) promoter linked to a ras oncogene. Mice transgenic for gamma GT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the gamma-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.

Similar articles

See all similar articles

Cited by 25 PubMed Central articles

See all "Cited by" articles

References

    1. J Biol Chem. 1990 Jul 15;265(20):11721-5 - PubMed
    1. Science. 1989 Sep 1;245(4921):971-3 - PubMed
    1. Cell. 1981 Nov;27(1 Pt 2):223-31 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2065-9 - PubMed
    1. Mol Biol Med. 1989 Aug;6(4):283-98 - PubMed

Publication types

LinkOut - more resources

Feedback