Abstract
Background:
Among Myc family genes, c-Myc is known to have a role in neural crest specification in Xenopus and in craniofacial development in the mouse. There is no information on the function of other Myc genes in neural crest development, or about any developmental role of zebrafish Myc genes.
Principal findings:
We isolated the zebrafish mych (myc homologue) gene. Knockdown of mych leads to severe defects in craniofacial development and in certain other tissues including the eye. These phenotypes appear to be caused by cell death in the neural crest and in the eye field in the anterior brain.
Significance:
Mych is a novel factor required for neural crest cell survival in zebrafish.
Publication types
-
Research Support, N.I.H., Intramural
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Biomarkers / metabolism
-
Body Patterning / drug effects
-
Brain / drug effects
-
Brain / metabolism
-
Branchial Region / abnormalities
-
Branchial Region / drug effects
-
Cell Death / drug effects
-
DNA, Complementary / isolation & purification
-
Embryo, Nonmammalian / abnormalities
-
Embryo, Nonmammalian / drug effects
-
Embryo, Nonmammalian / metabolism
-
Gene Expression Regulation, Developmental / drug effects
-
Molecular Sequence Data
-
Neural Crest / embryology*
-
Neural Plate / cytology
-
Neural Plate / drug effects
-
Oligonucleotides, Antisense / pharmacology
-
Phenotype
-
Sequence Analysis, Protein
-
Somites / abnormalities
-
Somites / drug effects
-
Transcription Factors / chemistry
-
Transcription Factors / genetics*
-
Transcription Factors / metabolism
-
Zebrafish / embryology*
-
Zebrafish / genetics*
-
Zebrafish Proteins / chemistry
-
Zebrafish Proteins / genetics*
-
Zebrafish Proteins / metabolism
Substances
-
Biomarkers
-
DNA, Complementary
-
Mych protein, zebrafish
-
Oligonucleotides, Antisense
-
Transcription Factors
-
Zebrafish Proteins