Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions

PLoS One. 2008 Apr 30;3(4):e2061. doi: 10.1371/journal.pone.0002061.

Abstract

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Affinity / methods*
  • DNA / metabolism
  • Humans
  • Protein Interaction Mapping / methods*
  • Proteins / metabolism*
  • Xenopus

Substances

  • Proteins
  • DNA