Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils

Methods Mol Biol. 2007;412:125-37. doi: 10.1007/978-1-59745-467-4_10.

Abstract

The measurement and manipulation of cytosolic free Ca2+ permits the investigation of the mechanisms of generation of the Ca2+ signal and cellular responses to these Ca2+ signals within living neutrophils. The optical methods most applicable to neutrophils, which will be discussed here, are (1) the use of fluorescent indicators of Ca2+ and (2) photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.

MeSH terms

  • Animals
  • Calcium / analysis*
  • Calcium / metabolism
  • Calcium Signaling
  • Cytosol / chemistry*
  • Fluorescent Dyes / pharmacokinetics
  • Fluorometry / methods
  • Humans
  • Neutrophils / chemistry*
  • Neutrophils / metabolism
  • Optics and Photonics*

Substances

  • Fluorescent Dyes
  • Calcium