Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-gamma and antibody responses in mice

Vaccine. 2008 Jun 2;26(23):2818-23. doi: 10.1016/j.vaccine.2008.03.080. Epub 2008 Apr 16.


Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluate the immunogenicity of these constructs, BALB/cJ mice were immunized twice with either Ad5.CS, Ad35.CS, empty Ad5-vector (eAd5), empty Ad35 vector (eAd35), or saline. Another group received the CSP-based RTS,S malaria vaccine formulated in the proprietary Adjuvant System AS01B (GlaxoSmithKline Biologicals, Rixensart, Belgium). Here we report that Ad5.CS, Ad35.CS, and RTS,S/AS01B, elicited both cellular and serologic CSP antigen-specific responses in mice. These adenoviral vectors induce strong malaria-specific immunity and warrant further evaluation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Antibodies, Protozoan / analysis
  • Antibodies, Protozoan / biosynthesis*
  • Antigens, Protozoan / genetics*
  • Antigens, Protozoan / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Genetic Vectors*
  • Immunization Schedule
  • Immunization, Secondary
  • Interferon-gamma / biosynthesis*
  • Malaria Vaccines / immunology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Plasmodium falciparum / genetics*
  • Plasmodium falciparum / metabolism*
  • Sporozoites / immunology*


  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Malaria Vaccines
  • Interferon-gamma