Molecular characterization of Enterococcus faecalis N06-0364 with low-level vancomycin resistance harboring a novel D-Ala-D-Ser gene cluster, vanL

Antimicrob Agents Chemother. 2008 Jul;52(7):2667-72. doi: 10.1128/AAC.01516-07. Epub 2008 May 5.

Abstract

Enterococcus faecalis N06-0364, exhibiting a vancomycin MIC of 8 microg/ml, was found to harbor a novel D-Ala-D-Ser gene cluster, designated vanL. The vanL gene cluster was similar in organization to the vanC operon, but the VanT serine racemase was encoded by two separate genes, vanTm(L) (membrane binding) and vanTr(L) (racemase).

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Composition
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecalis / genetics*
  • Enterococcus faecalis / isolation & purification
  • Genes, Bacterial*
  • Humans
  • Molecular Sequence Data
  • Multigene Family*
  • Operon
  • Peptide Synthases / genetics
  • Sequence Homology, Amino Acid
  • Vancomycin Resistance / genetics

Substances

  • Bacterial Proteins
  • DNA Primers
  • DNA, Bacterial
  • Peptide Synthases
  • VanC protein, Enterococcus