An experimental test of stroke recovery by implanting a hyaluronic acid hydrogel carrying a Nogo receptor antibody in a rat model

Biomed Mater. 2007 Dec;2(4):233-40. doi: 10.1088/1748-6041/2/4/005. Epub 2007 Oct 8.

Abstract

The objective of the study was to determine the effects of a hyaluronic-acid-based (HA-based) hydrogel implant, carrying a polyclonal antibody to the Nogo-66 receptor (NgR), on adult rats that underwent middle cerebral artery occlusion (MCAO). Behavioral tests of a forelimb-reaching task suggested that the disabled function of the impaired forelimb in this stroke model was ameliorated by the implant to a certain extent. These behavioral findings were correlated with immunohistochemical results of investigating the distribution of NgR antibody, neurofilaments (NF) and neuron-specific class III beta-tubulin (TuJ1) in the brain sections. The porous hydrogel functioned as a scaffold to deliver the NgR antibody, support cell migration and development. In addition, it was found NF-positive and TuJ1-positive expressions were distributed in the implanted hydrogel. Collectively, the results demonstrate the promise of the HA hydrogel as a scaffold material and the delivery vehicle of the NgR antibody for the repair of defects and the support of neural regeneration in the brain.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / administration & dosage*
  • Antibodies, Monoclonal / chemistry
  • Disease Models, Animal*
  • Drug Implants / chemistry*
  • GPI-Linked Proteins
  • Hyaluronic Acid / chemistry*
  • Hydrogels / chemistry
  • Myelin Proteins / immunology*
  • Nogo Receptor 1
  • Paresis / drug therapy*
  • Paresis / etiology
  • Rats
  • Receptors, Cell Surface / immunology*
  • Recovery of Function / drug effects*
  • Stroke / complications
  • Stroke / drug therapy*
  • Treatment Outcome

Substances

  • Antibodies, Monoclonal
  • Drug Implants
  • GPI-Linked Proteins
  • Hydrogels
  • Myelin Proteins
  • Nogo Receptor 1
  • RTN4R protein, human
  • Receptors, Cell Surface
  • Hyaluronic Acid