Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jan;65(1):415-23.

Epstein-Barr Virus Nuclear Antigen 2 Activates Transcription of the Terminal Protein Gene

Affiliations
Free PMC article

Epstein-Barr Virus Nuclear Antigen 2 Activates Transcription of the Terminal Protein Gene

U Zimber-Strobl et al. J Virol. .
Free PMC article

Abstract

Transcription of the terminal protein (TP) gene of Epstein-Barr virus (EBV) in Burkitt's lymphoma cells, in EBV-negative Burkitt's lymphoma cells converted with transformation-defective (P3HR1) and transformation-competent (B95-8, AG876) EBV strains, and in EBV-immortalized cell lines was studied. A TP1 cDNA probe spanning the boundary between exons 1 and 2 and discriminating between TP1 and TP2 transcripts was used for S1 analysis. TP RNA expression varied widely in Burkitt's lymphoma cells. TP-specific transcripts were not detectable or only hardly detectable in Burkitt's lymphoma cells with the group I phenotype (CD10+ CD77+ CD21- CD23- CD30- CDw70-) as well as in P3HR1 virus-converted Burkitt's lymphoma lines. TP expression was high in Burkitt's lymphoma lines with the group II and group III phenotypes (CD21+ CD23+ CD30+ CDw70+), in B95-8 and AG876 virus-converted lines, and in EBV-immortalized cells. Detection of TP1 RNA correlated with EBNA2 expression. TP1 transcription was shown to be dependent on EBNA2 expression by stable transfection of an EBNA2 expression vector into P3HR1 virus-converted BL41 cells. EBNA2 is activating the TP1 as well as the TP2 promoter, as shown by the analysis of TP promoter-chloramphenicol acetyltransferase constructs transiently transfected into EBNA2-positive and EBNA2-negative Burkitt's lymphoma cells.

Similar articles

See all similar articles

Cited by 85 articles

See all "Cited by" articles

References

    1. Cell. 1988 Jan 15;52(1):51-61 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Nov;84(22):8060-4 - PubMed
    1. IARC Sci Publ. 1985;(60):309-18 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 May;84(10):3452-6 - PubMed
    1. Nature. 1989 Aug 3;340(6232):393-7 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback