Preventive effect of TRPV1 agonists capsaicin and resiniferatoxin on ischemia/reperfusion-induced renal injury in rats

J Cardiovasc Pharmacol. 2008 May;51(5):513-20. doi: 10.1097/FJC.0b013e31816f6884.


We evaluated the effect of capsaicin, one of the transient receptor potential vanilloid receptor 1 (TRPV1) agonists, on ischemic acute renal failure (ARF) in rats. Ischemic ARF was induced by occlusion of the left renal artery and vein for 45 minutes followed by reperfusion, 2 weeks after contralateral nephrectomy. Renal function in vehicle-treated ARF rats markedly decreased at 24 hours after reperfusion. Treatment with capsaicin (3, 10, and 30 mg/kg, orally) 30 minutes before ischemia dose-dependently attenuated ischemia/reperfusion-induced renal dysfunction. In renal tissues exposed to ischemia/reperfusion, neutrophil infiltration, renal superoxide production, and renal tumor necrosis factor (TNF)-alpha mRNA expression were augmented, but these alterations were attenuated by the treatment with capsaicin. On the other hand, ischemia/reperfusion-enhanced renal interleukin (IL)-10 mRNA expression and plasma concentrations of IL-10 were augmented by treatment with capsaicin in ARF rats. In addition, resiniferatoxin (20 microg/kg, subcutaneous), a more selective and potent TRPV1 agonist, showed a renoprotective effect on ischemia/reperfusion-induced renal injury, in a qualitatively similar way to cases seen with capsaicin. These results demonstrate that TRPV1 agonists prevent ischemia/reperfusion-induced renal dysfunction. These renoprotective effects seem to be closely related to the inhibition of inflammatory response via TRPV1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Kidney Injury / prevention & control*
  • Animals
  • Capsaicin / metabolism
  • Capsaicin / pharmacology
  • Capsaicin / therapeutic use*
  • Diterpenes / metabolism
  • Diterpenes / pharmacology
  • Diterpenes / therapeutic use*
  • Interleukin-10 / metabolism
  • Male
  • Neutrophil Infiltration / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / prevention & control*
  • TRPV Cation Channels / agonists*
  • Tumor Necrosis Factor-alpha / metabolism


  • Diterpenes
  • TRPV Cation Channels
  • TRPV1 receptor
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • resiniferatoxin
  • Capsaicin