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, 173 (2), 896-9

Transposon Tn5supF-based Reverse Genetic Method for Mutational Analysis of Escherichia Coli With DNAs Cloned in Lambda Phage

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Transposon Tn5supF-based Reverse Genetic Method for Mutational Analysis of Escherichia Coli With DNAs Cloned in Lambda Phage

S H Phadnis et al. J Bacteriol.

Abstract

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

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