Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence

Nature. 1991 Jan 24;349(6307):305-10. doi: 10.1038/349305a0.

Abstract

Voltage-dependent potassium, sodium and calcium ion channels may share a common mechanism of activation, in which the conserved S4 sequence acts as the primary voltage sensor. Site-directed mutagenesis of the S4 sequence of the Shaker potassium channel and electrophysiological analysis suggest that voltage-dependent activation involves the S4 sequence but is not solely due to electrostatic interactions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium Channels / chemistry
  • Cloning, Molecular
  • Drosophila melanogaster / genetics*
  • Electric Conductivity
  • Electrochemistry
  • Electrophysiology
  • Ion Channel Gating / physiology
  • Mutagenesis, Site-Directed*
  • Oocytes / metabolism
  • Potassium Channels / chemistry
  • Potassium Channels / genetics*
  • Potassium Channels / physiology
  • Protein Conformation
  • Sequence Homology, Nucleic Acid
  • Sodium Channels / chemistry
  • Structure-Activity Relationship
  • Transfection
  • Xenopus

Substances

  • Calcium Channels
  • Potassium Channels
  • Sodium Channels