Nanocapsules containing hinokitiol (HKL) were prepared by an emulsion-diffusion method. In an emulsification step in preparing nanocapsules, cetyltrimethylamonium chloride (CTAC) was employed as a cationic emulsifier, Poly(epsilon-caprolactone) (PCL) was use as a wall material and HKL dissolved in octylsalicylate (OS) was used as a core material. The submicron-sized nanoparticle was observed on a TEM. The size ranged 55-234 nm and the mean diameters were 223 nm, which were determined by a dynamic light scattering method. According to the results of pH-dependent microelectrophoresis, the absolute value of the surface potential of the nanocapsules was greater than 20 mV. The nanocapsules were colloidally stable over the pH range of 3-11. The nanocapsules were included in two kinds of preparations, namely shampoo and hair tonic, and the preparations were applied every day for 3 weeks on the clipped backs of 6 week-old mouse (C57BL/6) to investigate the hair growth-promoting effect. The degree of hair growth was evaluated by image-analysing the photographs of the backs and, in parallel, by the histological observation of the formation and the growth of hair or hair bulbs. The results were compared with those of commercially available Minoxidil solution (3%). Phosphate buffered saline was used as a control. The in vivo hair growth-promoting effects of the two preparations were comparable to those of Minoxidil solution. These results are in a good agreement with the histological and structural changes of follicles of the model animals, of which skins were treated with either the testing samples or the control in the same way the experiments of in vivo hair growth promotion were performed.