A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase

Biochem J. 1991 Jan 1;273(Pt 1)(Pt 1):63-6. doi: 10.1042/bj2730063.

Abstract

A monoclonal antibody has been developed against the type II PtdIns 4-kinase from bovine brain. This antibody, 4C5G, causes greater than 90% inhibition of the type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. However, it fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver. These results suggest that type II and type III PtdIns 4-kinases are distinct gene products, and that 4C5G will be useful in studying the function of the type II PtdIns 4-kinase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Phosphatidylinositol 4-Kinase
  • Animals
  • Antibodies, Monoclonal*
  • Brain / enzymology
  • Cattle
  • Erythrocytes / enzymology
  • Humans
  • Liver / enzymology
  • Ovum / enzymology
  • Phosphotransferases / antagonists & inhibitors
  • Phosphotransferases / immunology*
  • Phosphotransferases / isolation & purification
  • Rats
  • Xenopus laevis

Substances

  • Antibodies, Monoclonal
  • Phosphotransferases
  • 1-Phosphatidylinositol 4-Kinase