Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes

J Virol Methods. 2008 Jul;151(1):158-60. doi: 10.1016/j.jviromet.2008.03.024. Epub 2008 May 12.

Abstract

Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added.

Publication types

  • Evaluation Study

MeSH terms

  • DNA Probes*
  • DNA, Viral / analysis
  • DNA, Viral / metabolism
  • Enzyme Stability
  • Herpes Simplex / virology
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / isolation & purification
  • Herpesvirus 2, Human / genetics*
  • Herpesvirus 2, Human / isolation & purification
  • Hot Temperature
  • Humans
  • Nucleic Acid Hybridization / methods*
  • Polymerase Chain Reaction / methods
  • Transition Temperature*
  • Uracil-DNA Glycosidase / metabolism*

Substances

  • DNA Probes
  • DNA, Viral
  • Uracil-DNA Glycosidase