Priming and stimulation of bovine neutrophils by recombinant human interleukin-1 alpha and tumor necrosis factor alpha

J Leukoc Biol. 1991 Feb;49(2):107-15. doi: 10.1002/jlb.49.2.107.

Abstract

We have examined the in vitro effects of two recombinant human monokines, interleukin-1 alpha (rHuIL-1 alpha) and tumor necrosis factor alpha (rHuTNF alpha), on bovine neutrophil functions. Both rHuIL-1 alpha (10 to 1,000 ng/ml) and rHuTNF alpha (5 to 50 ng/ml) directly stimulated the oxidative burst of bovine neutrophils as measured by Luminol-dependent chemiluminescence, superoxide anion generation, and hydrogen peroxide production. In addition, both rHuIL-1 alpha (1 to 1,000 ng/ml) and rHuTNF alpha (0.5 to 50 ng/ml) primed bovine neutrophils for an enhanced oxidative burst to subsequent stimulation with opsonized zymosan. Neutrophils pre-treated with either monokine exhibited an earlier, as well as stronger, zymosan-stimulated Luminol-dependent chemiluminescence response, as compared to untreated neutrophils. Exposure of bovine neutrophils to combinations of suboptimal doses of rHuIL-1 alpha (10 and 100 ng/ml) and rHuTNF alpha (0.5 and 5 ng/ml) resulted in a synergistic stimulation of Luminol-dependent chemiluminescence, whereas, no synergism was observed when using optimal doses of each monokine. Pre-incubation of bovine neutrophils with an optimal concentration of recombinant bovine interferon gamma (100 U/ml), and either rHuIL-1 alpha or rHuTNF alpha, further augmented the maximal oxidative response of neutrophils stimulated with opsonized zymosan. Bovine neutrophils released both primary and secondary granules in response to rHuIL-1 alpha and rHuTNF alpha, and also exhibited enhanced adherence in the presence of either monokine.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cell Adhesion / drug effects
  • Cytoplasmic Granules / drug effects
  • Cytoplasmic Granules / ultrastructure
  • In Vitro Techniques
  • Interleukin-1 / pharmacology*
  • Kinetics
  • Luminescent Measurements
  • Luminol
  • Neutrophils / cytology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Recombinant Proteins / pharmacology
  • Superoxides / blood
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Interleukin-1
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Superoxides
  • Luminol