Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc

Silvia Rossbach; Danielle J Mai; Eric L Carter; Laurent Sauviac; Delphine Capela; Claude Bruand; Frans J de Bruijn

doi:10.1128/AEM.02244-07

Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc

Appl Environ Microbiol. 2008 Jul;74(13):4218-21. doi: 10.1128/AEM.02244-07. Epub 2008 May 9.

Authors

Silvia Rossbach¹, Danielle J Mai, Eric L Carter, Laurent Sauviac, Delphine Capela, Claude Bruand, Frans J de Bruijn

Affiliation

¹ Department of Biological Sciences, Western Michigan University, Kalamazoo, MI 49008-5410, USA. Silvia.Rossbach@wmich.edu

Abstract

Whole-genome transcriptional profiling was used to identify genes in Sinorhizobium meliloti 1021 that are differentially expressed during exposure to elevated concentrations of cadmium and zinc. Mutant strains with insertions in metal-regulated genes and in genes encoding putative metal efflux pumps were analyzed for their metal sensitivities, revealing a crucial role for the SMc04128-encoded P-type ATPase in the defense of S. meliloti against cadmium and zinc stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Cadmium / pharmacology*
Dose-Response Relationship, Drug
Gene Expression Profiling*
Gene Expression Regulation, Bacterial
Genome, Bacterial
Heat-Shock Response
Mutation
Sinorhizobium meliloti / drug effects*
Sinorhizobium meliloti / genetics
Sinorhizobium meliloti / metabolism
Zinc / pharmacology*

Substances

Bacterial Proteins
Cadmium
Zinc