Extra centrosomes and/or chromosomes prolong mitosis in human cells

Nat Cell Biol. 2008 Jun;10(6):748-51. doi: 10.1038/ncb1738. Epub 2008 May 11.


Using laser microsurgery and cell fusion we have explored how additional centrosomes and/or chromosomes influence the duration of mitosis in human cells. We found that doubling the chromosome number added approximately 10 min to a 20 min division, whereas doubling the number of centrosomes added approximately 30 min more. Extra centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the spindle assembly checkpoint. Thus mitosis can be prolonged by non-genetic means and extra chromosomes and centrosomes probably contribute to the elevated mitotic index seen in many tumours.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Cycle
  • Cell Line
  • Cell Nucleus / metabolism
  • Centrosome / metabolism
  • Centrosome / ultrastructure*
  • Chromosomes / metabolism
  • Chromosomes / ultrastructure*
  • Humans
  • Kinetochores / ultrastructure
  • Microscopy, Video
  • Microtubules / chemistry
  • Mitosis*
  • Models, Biological
  • Spindle Apparatus
  • Time Factors