A genetic linkage map of Brassica nigra, comprised of 288 loci in eight linkage groups, was constructed. The linkage groups varied in size from 72 to 159 cM and the total map length was 855 cM. The recurrent parent used in the backcross was extremely heterozygous. This allowed recombination to be estimated separately for female (recurrent parent) meiosis and male (F1) meiosis over a large proportion of the genome. Significant differences between male and female recombination frequencies were observed on all six linkage groups where data was available for both sexes. Enhanced male recombination frequencies were observed that were associated with proterminal regions, while enhanced female recombination frequencies were adjacent to putative centromeres. It is possible that the distinct genotypes of the F1 (male) and recurrent (female) parents contributed to the observed differences in recombination. However, this study emphasizes the need to consider potential sex differences, in both the rate and the position of recombination, when planning genetic experiments and breeding programmes.