Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene

FEBS Lett. 1991 Jan 14;278(1):107-14. doi: 10.1016/0014-5793(91)80095-k.


We have characterized overlapping cDNA clones encoding cGMP phosphodiesterase (PDE) alpha- and beta-subunits of mouse retinal rod photoreceptors. The open reading frames predict an alpha-subunit of 100 kDa (856 residues), and a beta-subunit of 99 kDa (853 residues). Sequence analysis of two of twelve beta-subunit clones predicts the presence in the retina of an additional PDE, termed beta', which is generated by alternative splicing of the beta-subunit gene. beta' differs from beta only at the C-terminus being 55 residues shorter and lacking the Caax motif found at the C-termini of both the alpha- and beta-subunits. A 300 residue segment thought to contain the active site is present in the C-terminal half of alpha, beta and beta'.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3',5'-Cyclic-GMP Phosphodiesterases / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA / genetics*
  • Introns
  • Isoenzymes / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Open Reading Frames
  • Photoreceptor Cells / enzymology*
  • Polymerase Chain Reaction
  • RNA Splicing


  • Isoenzymes
  • DNA
  • 3',5'-Cyclic-GMP Phosphodiesterases

Associated data

  • GENBANK/M60451
  • GENBANK/X58796
  • GENBANK/X58797
  • GENBANK/X58798
  • GENBANK/X58799
  • GENBANK/X58800
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X60664