Selection of phage-displayed peptides that bind to a particular ligand-bound antibody

Bioorg Med Chem. 2008 Jun 1;16(11):5926-31. doi: 10.1016/j.bmc.2008.04.062. Epub 2008 Apr 27.


Phage-displayed peptides that selectively bind to aldolase catalytic antibody 93F3 when bound to a particular 1,3-diketone hapten derivative have been developed using designed selection strategies with libraries containing 7-12 randomized amino acid residues. These phage-displayed peptides discriminated the particular 93F3-diketone complex from ligand-free 93F3 and from 93F3 bound to other 1,3-diketone hapten derivatives. By altering the selection procedures, phage-displayed peptides that bind to antibody 93F3 in the absence of 1,3-diketone hapten derivatives have also been developed. With using these phage-displayed peptides, ligand-bound states of the antibody were distinguished from each other. A docking model of one of the peptides bound to the antibody 93F3-diketone complex was created using a sequential divide-and-conquer peptide docking strategy; the model suggests that the peptide interacts with both the antibody and the ligand through a delicate hydrogen bonding network.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Catalytic / chemistry
  • Antibodies, Catalytic / genetics
  • Antibodies, Catalytic / metabolism*
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / metabolism
  • Bacteriophage M13 / chemistry
  • Bacteriophage M13 / metabolism*
  • Binding Sites, Antibody*
  • Catalase / immunology
  • Catalase / metabolism*
  • Humans
  • Ketones / chemistry
  • Ketones / metabolism
  • Ligands
  • Mice
  • Mutant Chimeric Proteins / chemistry
  • Mutant Chimeric Proteins / genetics
  • Mutant Chimeric Proteins / metabolism
  • Oligopeptides / chemistry*
  • Oligopeptides / immunology
  • Oligopeptides / metabolism*
  • Peptide Library


  • Antibodies, Catalytic
  • Antibodies, Monoclonal
  • Ketones
  • Ligands
  • Mutant Chimeric Proteins
  • Oligopeptides
  • Peptide Library
  • Catalase