Circulating endothelial microparticles correlate inversely with endothelial function in patients with ischemic left ventricular dysfunction

J Card Fail. 2008 May;14(4):336-40. doi: 10.1016/j.cardfail.2007.11.002.


Background: Bone-marrow derived endothelial progenitor cells (CD34+ and VEGFR2+ KDR+ EPC) and endothelial-derived microparticles (CD 31+Annexin V+, EMP; indicator for endothelial apoptosis) were examined in the peripheral blood of 35 male, clinically stable patients with 3-vessel coronary artery disease (CAD). The patients were divided in 2 groups, those with preserved or normal function (n = 17; EF 65 +/- 6%) and those with reduced left ventricular (LV) function (n = 18; EF 36 +/- 11%).

Methods and results: The number of circulating EPCs was decreased by 25% (P = .07) and the number of EMPs was increased by 109 % (P < .05) in patients with LV dysfunction compared with those with normal or preserved LV function. EPCs were positively correlated (r = 0.24 for patients with LV dysfunction and r = 0.28 for patients with preserved LV function) with endothelial function as assessed by flow-mediated vasodilatation. In contrast, EMPs were inversely correlated (r = -0.42 for patients with LV dysfunction and r = -0.49 for patients with preserved LV function).

Conclusions: CAD patients with significant LV dysfunction show an increased index of endothelial cell damage. This decrease (or lack of compensatory elevation) of EPCs may result in a reduced potential for repair and thus contribute at least in part to the pathogenesis of endothelial dysfunction.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Annexin A5 / blood*
  • Coronary Artery Disease / blood
  • Coronary Artery Disease / physiopathology
  • Endothelial Cells*
  • Endothelium, Vascular / physiopathology*
  • Flow Cytometry
  • Humans
  • Male
  • Middle Aged
  • Myocardial Ischemia / blood*
  • Myocardial Ischemia / physiopathology*
  • Platelet Endothelial Cell Adhesion Molecule-1 / blood*
  • Risk Factors
  • Stem Cells
  • Stroke Volume
  • Ultrasonography
  • Ventricular Dysfunction, Left / blood
  • Ventricular Dysfunction, Left / diagnostic imaging
  • Ventricular Dysfunction, Left / physiopathology*


  • Annexin A5
  • Platelet Endothelial Cell Adhesion Molecule-1