[Analysis of EBV-LMP1 gene mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing]

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Feb;22(4):153-6.
[Article in Chinese]

Abstract

Objective: To detect and analyze the EBV-LMP1 gene N terminus XhoI-site mutation and C terminus 30 bp deletion in plasma of patients with nasopharyngeal carcinoma (NPC) in Chongqing of China.

Method: DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China. All the PCR production of N terminus was digested by enzyme XhoI, then segregated in 8% PAGE. All- the PCR production of C terminus was segregated in 8% PAGE too. Compared with B95-8 cell, some of the PCR production were sequenced and analyzed with software.

Result: LMP1 was amplified successfully from 48 of 48 NPC cases (100%) and from 38 of 40 non-NPC cases (95%) by PCR, but none of LMP1 XhoI-site mutation and none of 30 bp deletion was found by digesting, segregating and Sequencing.

Conclusion: EBV-LMP1 gene N terminus XhoI-site and C terminus 30 bp deletion have no mutation in plasma of NPC or non-NPC cases in Chongqing of China. The precise relationship of EBV-LMP1 gene mutation or deletion with NPC pathogenesis needs further investigation.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • China
  • DNA, Viral / genetics
  • Female
  • Herpesvirus 4, Human
  • Humans
  • Male
  • Middle Aged
  • Nasopharyngeal Neoplasms / blood
  • Nasopharyngeal Neoplasms / genetics
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / virology*
  • Sequence Deletion
  • Viral Matrix Proteins / blood*
  • Viral Matrix Proteins / genetics*

Substances

  • DNA, Viral
  • EBV-associated membrane antigen, Epstein-Barr virus
  • Viral Matrix Proteins