Small amplicon genotyping using internal temperature calibration and high resolution-melting

Biotechniques. 2008 Apr;44(4):577-8. doi: 10.2144/000112882.
No abstract available

MeSH terms

  • Base Sequence
  • Calibration
  • DNA / genetics
  • Fluorescent Dyes
  • Genotype
  • Heterozygote
  • Homozygote
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Denaturation*
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Thermodynamics
  • Transition Temperature*

Substances

  • Fluorescent Dyes
  • Oligonucleotide Probes
  • DNA