The Predikin webserver: improved prediction of protein kinase peptide specificity using structural information

Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W286-90. doi: 10.1093/nar/gkn279. Epub 2008 May 13.


The Predikin webserver allows users to predict substrates of protein kinases. The Predikin system is built from three components: a database of protein kinase substrates that links phosphorylation sites with specific protein kinase sequences; a perl module to analyse query protein kinases and a web interface through which users can submit protein kinases for analysis. The Predikin perl module provides methods to (i) locate protein kinase catalytic domains in a sequence, (ii) classify them by type or family, (iii) identify substrate-determining residues, (iv) generate weighted scoring matrices using three different methods, (v) extract putative phosphorylation sites in query substrate sequences and (vi) score phosphorylation sites for a given kinase, using optional filters. The web interface provides user-friendly access to each of these functions and allows users to obtain rapidly a set of predictions that they can export for further analysis. The server is available at

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Internet
  • Peptides / chemistry*
  • Peptides / metabolism
  • Phosphorylation
  • Protein Kinases / chemistry*
  • Protein Kinases / classification
  • Protein Kinases / metabolism
  • Sequence Analysis, Protein
  • Software*
  • Substrate Specificity


  • Peptides
  • Protein Kinases