Abstract
The complementary DNA of a metabotropic glutamate receptor coupled to inositol phosphate/Ca2+ signal transduction has been cloned and characterized. This receptor shows no sequence similarity to conventional G protein-coupled receptors and has a unique structure with large hydrophilic sequences at both sides of seven putative membrane-spanning domains. Abundant expression of this messenger RNA is observed in neuronal cells in hippocampal dentate gyrus and CA2-3 and in cerebellar Purkinje cells, suggesting the importance of this receptor in specific hippocampal and cerebellar functions.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cerebellum / metabolism
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Cloning, Molecular
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Electrophysiology / methods
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Female
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Glutamates / metabolism
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Glutamates / pharmacology*
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Models, Structural
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Oocytes / drug effects
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Oocytes / physiology
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Protein Conformation
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Rats
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Receptors, Glutamate
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Receptors, Neurotransmitter / drug effects
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Receptors, Neurotransmitter / genetics*
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Receptors, Neurotransmitter / physiology
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Sequence Homology, Nucleic Acid
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Xenopus
Substances
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Glutamates
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RNA, Messenger
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Receptors, Glutamate
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Receptors, Neurotransmitter