A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses

Immunity. 2008 May;28(5):639-50. doi: 10.1016/j.immuni.2008.03.017.

Abstract

B cells mediate multiple functions that influence immune and inflammatory responses. In this study, T cell-mediated inflammation was exaggerated in CD19-deficient (Cd19(-/-)) mice and wild-type mice depleted of CD20(+) B cells, whereas inflammation was substantially reduced in mice with hyperactive B cells as a result of CD19 overexpression (hCD19Tg). These inflammatory responses were negatively regulated by a unique CD1d(hi)CD5(+) B cell subset that was absent in Cd19(-/-) mice, represented only 1%-2% of spleen B220(+) cells in wild-type mice, but was expanded to approximately 10% of spleen B220(+) cells in hCD19Tg mice. Adoptive transfer of these CD1d(hi)CD5(+) B cells normalized inflammation in wild-type mice depleted of CD20(+) B cells and in Cd19(-/-) mice. Remarkably, IL-10 production was restricted to this CD1d(hi)CD5(+) B cell subset, with IL-10 production diminished in Cd19(-/-) mice, yet increased in hCD19Tg mice. Thereby, CD1d(hi)CD5(+) B cells represent a unique subset of potent regulatory B cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD1 / analysis
  • Antigens, CD1 / immunology
  • Antigens, CD1d
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocyte Subsets / metabolism
  • CD5 Antigens / analysis
  • CD5 Antigens / immunology
  • Cytokines / immunology
  • Cytokines / metabolism*
  • Immunophenotyping
  • Inflammation / immunology*
  • Inflammation / metabolism
  • Interleukin-10 / immunology
  • Interleukin-10 / metabolism*
  • Lymphocyte Depletion
  • Mice
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Peritoneal Cavity / cytology
  • Phenotype
  • Spleen / cytology
  • Spleen / immunology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism

Substances

  • Antigens, CD1
  • Antigens, CD1d
  • CD5 Antigens
  • Cytokines
  • Interleukin-10